dxa bone scan icon Search Results


inhibitor control (icon)  (Ribobio co)
90
Ribobio co inhibitor control (icon)
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Inhibitor Control (Icon), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/pmc10283203-74-22-28?v=Ribobio+co
Average 90 stars, based on 1 article reviews
inhibitor control (icon) - by Bioz Stars, 2026-08
90/100 stars
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icon2 sys system  (Bruker Corporation)
96
Bruker Corporation icon2 sys system
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Icon2 Sys System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/10__1364_slash_oe__386744-36-8-11?v=Bruker+Corporation
Average 96 stars, based on 1 article reviews
icon2 sys system - by Bioz Stars, 2026-08
96/100 stars
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icon arrays  (Trevena Inc)
90
Trevena Inc icon arrays
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Icon Arrays, supplied by Trevena Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/10__1002_slash_bdm__2341-51-0-29?v=Trevena+Inc
Average 90 stars, based on 1 article reviews
icon arrays - by Bioz Stars, 2026-08
90/100 stars
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icos  (Sino Biological)
93
Sino Biological icos
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Icos, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/custom%40icos%4038232701?v=Sino+Biological
Average 93 stars, based on 1 article reviews
icos - by Bioz Stars, 2026-08
93/100 stars
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ipod module  (apple inc)
90
apple inc ipod module
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Ipod Module, supplied by apple inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/us12277935-450-48-117?v=apple+inc
Average 90 stars, based on 1 article reviews
ipod module - by Bioz Stars, 2026-08
90/100 stars
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mir-874-3p mimic control  (Ribobio co)
90
Ribobio co mir-874-3p mimic control
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Mir 874 3p Mimic Control, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/pmc08939068-65-2-9?v=Ribobio+co
Average 90 stars, based on 1 article reviews
mir-874-3p mimic control - by Bioz Stars, 2026-08
90/100 stars
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inhibitor-control  (Ribobio co)
90
Ribobio co inhibitor-control
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Inhibitor Control, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/pm30894209-65-14-19?v=Ribobio+co
Average 90 stars, based on 1 article reviews
inhibitor-control - by Bioz Stars, 2026-08
90/100 stars
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pemetrexed  (FUJIFILM)
90
FUJIFILM pemetrexed
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Pemetrexed, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/pm32382354-63-7-27?v=FUJIFILM
Average 90 stars, based on 1 article reviews
pemetrexed - by Bioz Stars, 2026-08
90/100 stars
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control inhibitor  (Shanghai GenePharma)
90
Shanghai GenePharma control inhibitor
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Control Inhibitor, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/10__2147_slash_jir__s403531-82-39-44?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
control inhibitor - by Bioz Stars, 2026-08
90/100 stars
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trichostatin a (tsa)  (Biomol GmbH)
90
Biomol GmbH trichostatin a (tsa)
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Trichostatin A (Tsa), supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/us09862685-942-0-8?v=Biomol+GmbH
Average 90 stars, based on 1 article reviews
trichostatin a (tsa) - by Bioz Stars, 2026-08
90/100 stars
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mimic control  (Ribobio co)
90
Ribobio co mimic control
CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus <t>iCon.</t> iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 <t>versus</t> <t>(Lsh-NC</t> + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)
Mimic Control, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dxa+bone+scan+icon/pmc08973981-47-2-25?v=Ribobio+co
Average 90 stars, based on 1 article reviews
mimic control - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus iCon. iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 versus (Lsh-NC + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)

Journal: Respiratory Research

Article Title: CircItgb5 promotes synthetic phenotype of pulmonary artery smooth muscle cells via interacting with miR-96-5p and Uba1 in monocrotaline-induced pulmonary arterial hypertension

doi: 10.1186/s12931-023-02480-9

Figure Lengend Snippet: CircItgb5 regulates mTOR expression through sponging miR-96-5p. A A cytoscape was used to visualize miR-96-5p-target genes interaction based on TargetScan. Six genes highlighted in purple were previously reported to be related to PH. B Expression of the selected six genes in PASMCs was determined by RT-qPCR. *0.01 ≤ P ≤ 0.05 versus mimic control. C , D The luciferase activities of wild-type (WT) or mutant (MUT) pGL3-mTOR 3'UTR in 293 T cells cotransfected with miR-96-5p mimic or mimic control. The red letter represents mutated sequences. The mutation principle: G to U, U to G, C to A, A to C. **0.001 ≤ P ≤ 0.009 versus mimic control. E mTOR expression was determined by western blotting after the corresponding treatment in PASMCs. **0.001 ≤ P ≤ 0.009 versus mimic control, # 0.01 ≤ P ≤ 0.05 versus iCon. iCon, inhibitor control. F mTOR expression was determined by western blotting after the miR-96-5p inhibitor rescue experiment in PASMCs. *0.01 ≤ P ≤ 0.05 versus (Lsh-NC + iCon), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon). G Relative mTOR level was detected in the lungs and PASMCs after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus control, # 0.01 ≤ P ≤ 0.05 versus Lsh-NC. All data are presented as means ± SD (n = 3 biological replicates)

Article Snippet: CircItgb5 lentiviral small hairpin RNA (Lsh-circItgb5), lentiviral small hairpin RNA negative control (Lsh-NC), miR-96-5p mimic, miR-96-5p inhibitor, a mimic control, and an inhibitor control (iCon) were synthesized by RiboBio (Guangdong, China).

Techniques: Expressing, Quantitative RT-PCR, Control, Luciferase, Mutagenesis, Western Blot

CircItgb5 promotes synthetic phenotype of PASMCs by interacting with miR-96-5p and Uba1. A , C Protein expression of COL1A1 and SM22α was determined after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus (Lsh-NC + iCon), **0.001 ≤ P ≤ 0.009 versus (Lsh-NC + vector), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon) or (Lsh-circItgb5 + vector), ## 0.001 ≤ P ≤ 0.009 versus (Lsh-circItgb5 + vector). B , D The cell cycle of PASMCs was measured by flow cytometry. *0.01 ≤ P ≤ 0.05 versus (Lsh-NC + iCon), **0.001 ≤ P ≤ 0.009 versus (Lsh-NC + vector), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon) or (Lsh-circItgb5 + vector). All data are presented as means ± SD (n = 3 biological replicates)

Journal: Respiratory Research

Article Title: CircItgb5 promotes synthetic phenotype of pulmonary artery smooth muscle cells via interacting with miR-96-5p and Uba1 in monocrotaline-induced pulmonary arterial hypertension

doi: 10.1186/s12931-023-02480-9

Figure Lengend Snippet: CircItgb5 promotes synthetic phenotype of PASMCs by interacting with miR-96-5p and Uba1. A , C Protein expression of COL1A1 and SM22α was determined after the corresponding treatment. *0.01 ≤ P ≤ 0.05 versus (Lsh-NC + iCon), **0.001 ≤ P ≤ 0.009 versus (Lsh-NC + vector), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon) or (Lsh-circItgb5 + vector), ## 0.001 ≤ P ≤ 0.009 versus (Lsh-circItgb5 + vector). B , D The cell cycle of PASMCs was measured by flow cytometry. *0.01 ≤ P ≤ 0.05 versus (Lsh-NC + iCon), **0.001 ≤ P ≤ 0.009 versus (Lsh-NC + vector), # 0.01 ≤ P ≤ 0.05 versus (Lsh-circItgb5 + iCon) or (Lsh-circItgb5 + vector). All data are presented as means ± SD (n = 3 biological replicates)

Article Snippet: CircItgb5 lentiviral small hairpin RNA (Lsh-circItgb5), lentiviral small hairpin RNA negative control (Lsh-NC), miR-96-5p mimic, miR-96-5p inhibitor, a mimic control, and an inhibitor control (iCon) were synthesized by RiboBio (Guangdong, China).

Techniques: Expressing, Plasmid Preparation, Flow Cytometry